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INTRODUCTION The need for an antihelmintic, with a broad spectrum of antiparasitic effect, highly efficient, with good safety limits and that would also be versatile in its administration, promoted a study of hundreds of benzimidazole compounds.Its in vitro antifungal activity was observed against Blastomyces spp, Aspergillus spp, Fusarium spp, Penicillium spp and Trichophyton spp, although there have been controversies regarding its effect against some yeasts, such as Candida sp. (26,30). Malassezia pachydermatis is a yeast that is considered a normal inhabitant and an opportunistic pathogen of the external.

The NCCLS (22) recommends the use of RPMI 1640 in antifungal tests with yeasts (Candida spp. and Cryptococcus spp.), although it has not yet been used with the Malassezia species (25).In the current literature there are no studies of thiabendazole and the adapted BM method to compare these results.

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The aim of this study was to evaluate the in vitro effect of thiabendazole against 51 isolates of M. pachydermatis using the CLSI Broth Microdilution method that has been adapted for this yeast species (NCCLS, 2002).Thiabendazole was the first compound that fulfilled all of those requirements with an extraordinary performance (26,27).

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Cell morphology was observed through the use of colony smears, which were stained with Gram stains and then characterized (15).Key words: Malassezia pachydermatis, Minimum Inhibitory Concentration, thiabendazole.

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Some authors recommend the evaluation of antifungal sensitivity through the use of antimycograms in special clinical conditions, such as cases of therapeutic failure, which is quite common in cases of canine external otitis and dermatitis exacerbated by M. pachydermatis (1,8,14).

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However, when comparing the susceptibility of M. pachydermatis against thiabendazole with other antifungals, the MIC has different values and the percentage of resistant isolates to this drug.

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Furthermore, there was no attempt to experimentally compare the current results with those from methods used and published in internationally peer reviewed journals or compare with drugs having MICs defined by these methods.The realization of the 5th International Seminar on Process Hydrometallurgy.Jan 14, 2015 - BSAC Methods for Antimicrobial Susceptibility Testing.

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Exposure to ethanol in Brazilian gasoline station attendants. Detection of ethanol in Brazilian gasoline station. specimen vo lume of 4 mL.This study used the agar diffusion method for testing this and other antifungal drugs against M. pachydermatis.In vitro testing of antimicrobial susceptibility is of great importance.

The lowest concentration that produced a significant inhibition (around 50%) of the growth of the yeast in comparison with the positive control was identified as the MIC.Sharma, R from ENGINEERIN 48840 at University of Technology.The onset of clinical disease occurs when there is excessive multiplication of M. pachydermatis due to alterations in the defense mechanisms of the host.

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The Buyers Guide is the official Buying Resource of the Mid-America Lumbermens Association (MLA), serving independent lumber and building material retailers in Arkansas, Kansas, Missouri and Oklahoma.The NCCLS Broth Microdilution (BM) method adapted for M. pachydermatis was performed by Eichenberg et al. and Nascente et al. (9,19) according to the M27A2 document (22) and thiabendazole recommendations.

Version 14. Page. Standing Committee members. 4. Changes in document. 5. Preface.In recent years, studies have also reported M. pachydermatis as the cause of canine dermatitis (4,6,21,24,28).

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The antifungals reported in the CLSI document (22) included the azoles (itraconazole, fluconazole and ketoconazole), 5-flucitosine and amphotericine.Wireless Instrumented Sphere For Three-Dimensional. - lume ufrgs.Create your citations, reference lists and bibliographies automatically using the APA, MLA, Chicago, or Harvard referencing styles.ML Talent Agency, Toronto, Ontario. 7.8K likes. Background Talent Agency ML Talent Agency specializes in background extras for Feature Films, Television.The M27A2 is a reference protocol that was written to provide consensus on the evaluation of the susceptibility of yeasts against antifungals.Resistance was verified in seven (13.7%) isolates of the yeast, intermediate sensitivity in 24 (47.1%) isolates and sensitivity in 20 (39.2%) strains (Table 1).





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